Separace amplikonů specifických pro rod Lactobacillus s využitím magnetického nosiče

• Title in English: Separation of amplicons specific for genus Lactobacillus by magnetic carrier
• Authors: JANOSZOWSKÁ Dagmar; ŠPANOVÁ Alena; RITTICH Bohuslav; HORÁK Daniel
• Year of publication: 2008
• Type: Article in Proceedings
• Conference: XII. Setkání biochemiků a molekulárních biologů
• MU Faculty or unit: Faculty of Science
• Field: Genetics and molecular biology
• Keywords: Lactobacillus; DNA isolation; magnetic microspheres; streptavidin
• Description: DNA isolated from bacterial cells of genus Lactobacillus was amplified using genus specific primers LbLMA1 a R-16-1 (Dubernet a spol., 2002). Primer R16-1 was biotinyled. Biotinyled PCR products were separated using magnetic carrier with immobilised streptavidin. The elution of PCR product from carrier was tested.

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