A simple procedure for isolation of PCR-ready bacterial DNA

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Authors

ŠPANOVÁ Alena RITTICH Bohuslav KŘÍŽOVÁ Jana NĚMCOVÁ Eva KORDAČOVÁ Lucie

Year of publication 2007
Type Article in Proceedings
Conference Ninth International Symposium on Advances in Extraction Technologies
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords DNA; isolation; salting-out; NaCl
Description PCR-ready DNA was isolated using salting-out procedure from crud cell lysates. The evaluated method is suitable for DNA isolation from pure cultures of bacterial cells. The reproducibility of RAPD and rep-PCR paterns was comparable with phenol extraction procedure.
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