Engineering of PA-IIL lectin from Pseudomonas aeruginosa - Unravelling the role of the specificity loop for sugar preference
| Title in English | ngineering of PA-IIL lectin from Pseudomonas aeruginosa - Unravelling the role of the specificity loop for sugar preference |
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| Authors | |
| Year of publication | 2007 |
| Type | Article in Periodical |
| Magazine / Source | BMC Structural Biology |
| MU Faculty or unit | |
| Citation | |
| Field | Biochemistry |
| Keywords | lectin; pseudomonas aeruginosa; protein engineering; microcalorimetry;X-ray |
| Description | Mutagenesis of amino acids forming the specificity binding loop in PA-IIL allowed identification of one amino acid that is crucial for definition of the lectin sugar preference. Altering specificity loop amino acids causes changes in saccharide-binding preferences of lectins derived from PA-IIL, via creation or blocking possible binding interactions. This finding opens a gate towards protein engineering and subsequent protein design to refine the desired binding properties and preferences, an approach that could have strong potential for drug design. |
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