Isolation of PCR-ready DNA from dairy products.

• Title in English: Isolation of PCR-ready DNA from dairy products
• Authors: RITTICH Bohuslav; ŠPANOVÁ Alena; PETROVÁ Kateřina; HORÁK Daniel
• Year of publication: 2004
• Type: Article in Proceedings
• Conference: New perspectives of probiotics
• MU Faculty or unit: Faculty of Science
• Field: Microbiology, virology
• Keywords: PCR; magnetic particles; dairy products; Bifidobacterium; Lactobacillus
• Description: Carboxyl group-containing magnetic nonporous P(HEMA-co-EDMA), P(HEMA-co-GMA)and PGMA microspheres were used for isolation of DNA from dairy products. Different poly(ethylene glycol) (PEG) and sodium chloride concentrations were used to optimize DNA adsorption. Proposed method was used for PCR identification of probiotic Bifidobacterium and Lactobacillus strains in different dairy products.

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