Enterococcus spp. associated with plants
| Authors | |
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| Year of publication | 2012 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | A group of 104 presumptive enterococcal strains was isolated on Kanamycin esculin azide agar in the frame of a study dealing with investigation of Enterococcus spp. populations occurring on plants. Isolated strains were analysed using rep-PCR with the (GTG)5 primer and obtained fingerprints were compared with an in-house CCM reference database containing more that 5000 fingerprints representing multiple Gram-positive bacterial species including all known Enterococcus spp. In total, 53 strains matched reference database entries and were identified as Enterococcus faecalis (18 strains), Enterococcus haemoperoxidus (13), Enterococcus casseliflavus (4), Enterococcus faecium (2), Enterococcus moraviensis (2), Enterococcus mundtii (2), and Enterococcus durans (1). Two groups of strains were assigned as recently proposed species Enterococcus ureilyticus (7) and Enterococcus rotai (4). Another group of initially unidentified eight plant associated strains was characterized in detail and described recently as Enterococcus plantarum. Remaining nine strains were identified as members of other genera (Lactococcus, Lactobacillus, Leuconostoc) or were not clustered with any fingerprints included in the database (34 strains). Overall results obtained in the frame of the present study revealed that plants are inhabited by diverse Enterococcus spp. A high proportion of unidentified strains and isolation of representatives of recently proposed Enterococcus spp. imply that unknown species occur within the taxonomically rich enterococcal populations associated with plants. |
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