Effects of Voltage-Sensitive Dye di-4-ANEPPS on Isolated Rat Heart Electrogram

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Authors	FIALOVÁ Kateřina KOLÁŘOVÁ Jana JANOUŠEK Oto RONZHINA Marina PROVAZNÍK Ivo NOVÁKOVÁ Marie
Year of publication	2011
Type	Article in Periodical
Magazine / Source	Computing in Cardiology
MU Faculty or unit	Faculty of Medicine
Citation
Web	http://cinc2011.org
Field	Cardiovascular diseases incl. cardiosurgery
Keywords	voltage-sensitive dye;isolated heart; rat;electrogram
Description	Although the classical suction microelectrodes are still considered golden standard for recording of monophasic action potentials, there are various new methods enabling to record electrical changes from small area on the heart surface. One of them is recording of cardiac electrical activity using voltage-sensitive dyes (VSDs) by optical method. VSDs exhibit large fluorescence and/or absorption changes that vary with changes of the membrane potential. Staining of the heart with the dye should not result in pharmacological or toxic effects to the preparation. Data about response of the heart to staining with VSD are scarce. In order to determine this response in classical biomodel in basic cardiology, we followed arrythmia-preceding parameters in isolated rat heart electrogram during staining with VSD di-4-ANEPPS and washout periods.
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