Kvantifikace proteinových biomarkerů pomocí hmotnostní spektrometrie pracující v režimu monitorování vybraných reakcí

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Title in English Mass-Spectrometry Quantification of Protein Biomarkers by Selected Reaction Monitoring
Authors

FAKTOR Jakub STRUHÁROVÁ Iva FUČÍKOVÁ Alena HUBÁLEK Martin VOJTĚŠEK Bořivoj BOUCHAL Pavel

Year of publication 2011
Type Article in Periodical
Magazine / Source Chemické listy
MU Faculty or unit

Faculty of Science

Citation
Field Biochemistry
Keywords protein quantification; mass spectrometry; proteomics
Description Selected reaction monitoring (SRM) is a quantitative MS-based method which recently expanded to analysis of proteins by determination of peptides. The instrumentation consists of a nanoflow liquid chromatograph coupled with nanoelectrospray ionization and a triple-quadrupole mass spectrometer. In principle, the first quadrupole works as a mass filter, transmitting only a selected peptide, which is fragmented in a second quadupole via collision-induced dissociation. The third quadrupole transmits the selected peptide fragments, which are then directed to the detector. This approach enables targeted quantification of tens of proteins in a single analysis with very good sensitivity and selectivity. The SRM becomes a key method in a new targeted-proteomics toolbox. The most important field of its applications lies in the life sciences research where SRM enables fast, accurate and cost-effective determination of proteins in large sets of samples.
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