c-Myb regulates MMP1, MMP9 and cathepsin D: Implications for the matrix-dependent breast cancer cell invasion and metastasis

• Authors: KNOPFOVÁ Lucia; BENEŠ Petr; PEKARČÍKOVÁ Lucie; HERMANOVÁ Markéta; MASAŘÍK Michal; SOUČEK Karel; ŠMARDA Jan
• Year of publication: 2011
• Type: Conference abstract
• MU Faculty or unit: Faculty of Science
• Description: Ectopically expressed c-Myb enhanced motility and ability of human MDA-MB-231 and mouse 4T1 mammary cancer cells to invade Matrigel but not the collagen I matrix in vitro. c-Myb strongly increased expression/activity of cathepsin D and matrix metalloproteinase (MMP) 9 and significantly down-regulated MMP1. The gene coding for cathepsin D was identified as direct c-Myb-target gene and downstream effector of the c-Myb migration-promoting function. Finally, we demonstrated that c-Myb delayed growth of mammary tumors in BALB/c mice and affected metastatic potential of breast cancer cells in an organ-specific manner. This study demonstrates potential role of a stromal context for the c-Myb function in control of breast cancer invasion and metastasis.

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