The X-ray structure versus NMR structure of delta-subunit of RNA-polymerase

Demo,  Gabriel; Papoušková,  Veronika; Sanderova, Hana; Žídek,  Lukáš; Wimmerová,  Michaela

The X-ray structure versus NMR structure of delta-subunit of RNA-polymerase

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Authors	DEMO Gabriel PAPOUŠKOVÁ Veronika SANDEROVA Hana ŽÍDEK Lukáš WIMMEROVÁ Michaela
Year of publication	2011
Type	Conference abstract
MU Faculty or unit	Central European Institute of Technology
Citation
Description	The N-terminal domain of delta subunit was conquested to high-throughput screening. Iodine compound was used as a co-crystalline substance to have a possibility to provide a Single Anomalous Dispersion (SAD) experiments. Diffraction data were collected at BESSY II Berlin, beamline MX-14.2. The alpha-helical part of the protein is in a good agreement with the NMR structure. In the region of beta-sheets for the NMR structure the X-ray structure showed completely different behaviour (folding).
Related projects:	Proteins in metabolism and interaction of organisms with the environment Delta podjednotka RNA polymerázy z Gram pozitivních bakterií