Nový lektin ze světélkující bakterie Photorhabdus luminescens
| Title in English | New lectin from bioluminescent bacterium Photorhabdus luminescens |
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| Authors | |
| Year of publication | 2011 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | The protein PLU from P. luminescens has a strong sequence homology with characterized lectin BC2L-C-nt from the human opportunistic pathogen Burkholderia cenocepacia. So far, PLU is the only one supposed homologous of BC2L-C-nt lectin. Function studies were performed using surface plasmon resonance and isothermal titration calorimetry, which allowed identifying binding properties and thermodynamic parameters of interaction with saccharides. Preliminary information about a quaternary structure of the protein was obtained using dynamic light scattering, crosslinking with bifunctional reagents and analytical ultracentrifugation. |
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