Development of a Crystallization Protocol for the DbeA1 Variant of Novel Haloalkane Dehalogenase from Bradyrhizobium elkani USDA94

Prudnikova,  Tanyana; Chaloupková,  Radka; Sato,  Yukari; Nagata,  Yuji; Degtjarik, O.; Kutý,  Michal; Rezacova, P.; Damborský,  Jiří; Kuta-Smatanová, Smatanova I.

Development of a Crystallization Protocol for the DbeA1 Variant of Novel Haloalkane Dehalogenase from Bradyrhizobium elkani USDA94

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Authors	PRUDNIKOVA Tanyana CHALOUPKOVÁ Radka SATO Yukari NAGATA Yuji DEGTJARIK O. KUTÝ Michal REZACOVA P. DAMBORSKÝ Jiří KUTA-SMATANOVÁ Smatanova I.
Year of publication	2011
Type	Article in Periodical
Magazine / Source	Crystal Growth & Design
MU Faculty or unit	Faculty of Science
Citation
Doi	http://dx.doi.org/10.1021/cg1013363
Field	Biochemistry
Keywords	Crystallization; X-ray;Haloalkane dehalogenases
Description	The DbeA1 variant of a novel haloalkane dehalogenaseDbeA (EC 3.8.1.5) from Bradyrhizobium elkani USDA94 was constructed to study the structure-function relationships between DbjA and DbeA enzymes. A DbeA1 variant carries a unique fragment of nine amino acids transplanted from the sequentially closely related enzyme DbjA from Bradyrhizobium japonicum USDA110 to DbeA. Here we report development of the crystallization protocol for DbeA1 and soaking experiments with the ligands 1-fluoropentane and 1,3-dichloropropane.
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