Fe/Mn superoxide dismutase in Paracoccus denitrificans is induced by azide and expressed independently on FNR-type regulators

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Authors

BOUCHAL Pavel VYHLÍDALOVÁ Tereza STRUHÁROVÁ Iva ZDRÁHAL Zbyněk KUČERA Igor

Year of publication 2011
Type Article in Periodical
Magazine / Source Folia Microbiologica
MU Faculty or unit

Faculty of Science

Citation
Web Full text
Doi http://dx.doi.org/10.1007/s12223-011-0007-3
Field Biochemistry
Keywords Superoxide dismutase; Paracoccus denitrificans; azide; FNR regulators; proteomics
Description In the facultatively anaerobic bacterium Paracoccus denitrificans, the synthesis of many proteins involved in energy metabolism is regulated by the FNR-type transcription factors (FnrP, NNR and NarR). One of them, NarR, senses nitrate or nitrite concentration and promotes expression of the membrane-bound nitrate reductase genes. It can also be activated by azide as a non-metabolizable analogue of nitrate. In this work we show that one of the most prominent protein species inducible by azide is a Fe/Mn-family superoxide dismutase (SOD). Azide induces SOD at protein, mRNA transcript and enzyme activity levels in the aerobically growing cells. Since SOD expression remains unaffected in the fnrP, nnr and narR mutant strains, we postulate a mechanism independent of the known FNR-type regulators but involving a redox signal arising from the respiratory chain.
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