Heavy metal status in prostate cancer
| Authors | |
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| Year of publication | 2011 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Prostate tissue is specific in zinc metabolism – it accumulates high level of zinc(II) – up to ten times higher compared to other tissues. In such concentration, zinc(II) affects energetic metabolism, antioxidative processes and apoptosis. However, prostate cancer has significantly lower intracellular level of zinc(II). Therefore, distinct behaviour of tumor cells is expected. This study focuses on impact of zinc(II) on following cell lines: (1) 22Rv1 (derived from a xenograft from androgen-dependent primary tumor of Gleason sum 9), (2) PNT1A (established by immortalisation of normal adult prostatic epithelial cells by transfection with SV40). Theese cell lines were fed with increasing concentration of zinc(II) in medium. Cell growth was monitored by real-time impedance monitoring system, mRNA and protein level of zinc-related and oxidative-stress-related genes were determined, level of intracellular zinc was deteremined using electrochemical methods. We found significant differences in expression of zinc-transporter genes and zinc-binding proteins between cell lines; we found significantly elevated levels of major zinc transporters in tumorous cell line in mRNA level and correlations between zinc(II) level in medium and level of zinc-binding protein metallothionein in cells. Based on obtained results, it can be concluded that zinc(II) may play a role in prostate cancer pathogenesis. |
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