SOLVING PHASE PROBLEM USING A SE-MET DERIVATIVE OF THE FLAVOENZYME NAD(P)H:ACCEPTOR OXIDOREDUCTASE (FERB)
| Authors | |
|---|---|
| Year of publication | 2010 |
| Type | Article in Proceedings |
| Conference | Materials Structure, vol. 17, no. 1a (2010) |
| MU Faculty or unit | |
| Citation | |
| Field | Biochemistry |
| Keywords | phase problem; flavoenzyme; FerB; reductase; crystallography |
| Description | The flavin adenine dinucleotide-dependent enzyme FerB from Paracoccus denitrificans reduces a range of sub strates, including chromate, ferric complexes, benzoquinones and naphtoquinones. The reduced form of nicotinamide adenine dinucleotide serves as a source of electrons. Recombinant unmodified and selenometionine-substituted (Se-Met) FerB derivatives were crystallized, diffraction data for both forms were collected and the phase problem for Se-Met FerB dimer was solved by three-wavelength multiple anomalous dispersion, followed by the combination of molecular replacement and single-wavelength anomalous diffraction phasing. A molecular replacement solution of unmodified FerB tetramer was obtained using Se-Met structure as a search model. |
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