Multiple myeloma patients at peripheral blood stem cell harvest: restricted usage of TCR beta variable families

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Authors

TESAŘOVÁ Lenka KRONTORÁD KOUTNÁ Irena KOŘÍSTEK Zdeněk KLABUSAY Martin

Year of publication 2011
Type Article in Periodical
Magazine / Source Clinical Immunology
MU Faculty or unit

Faculty of Informatics

Citation
Doi http://dx.doi.org/10.1016/j.clim.2010.09.007
Field Genetics and molecular biology
Keywords T-cell receptor; Beta variable families; Multiple myeloma; Reverse transcription polymerase chain reaction; Capillary electrophoresis
Description The immune systems of multiple myeloma patients are suppressed by the disease itself, and this immunosuppression is further enhanced by standard therapies. The aim of our study was to evaluate the effects of initial chemotherapy and a peripheral blood mobilisation regimen on T-cell population diversity. Reverse transcription polymerase chain reaction (RT-PCR) with a new set of primers, in combination with capillary electrophoresis, was established. The methodology was used to analyse the relative expression of 27 T-cell receptor beta variable gene families (BV families) in multiple myeloma patients undergoing peripheral blood stem cell harvest. We found that the overall BV family usage in these patients was restricted; the relative expression of 10 BV families was significantly depressed in patients compared to healthy donors. These findings demonstrate that the preparative regimen for autologous stem cell transplantation affects the T-cell population in terms of the restriction of its T-cell receptor diversity.
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