Ethanol-fixed material used for both classical and molecular identification purposes: Eudiplozoon nipponicum (Monogenea: Diplozoidae) as a case parasite species

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Authors

KOŠKOVÁ Eva MATĚJUSOVÁ Iveta CIVÁŇOVÁ Kristína KOUBKOVÁ Božena

Year of publication 2010
Type Article in Periodical
Magazine / Source Parasitology Research
MU Faculty or unit

Faculty of Science

Citation
Web http://www.springerlink.com/content/817124kpr63556u1/
Field Zoology
Keywords sodium dodecyl sulphat;ethanol fixed material; Diplozoidae
Description This study is focused on the feasibility of two treatments of alcohol-fixed monogenean parasites which are intended to be use for the combined morphological and molecular characterizations. The monogenean parasite, Eudiplozoon nipponicum, was selected as a model parasite species; however it is expected that these techniques will be suitable for other monogeneans and other parasitic families. The haptor of diplozoid parasites is equipped with sclerotized attachment clamps and central hooks which are utilized for morphological identification. As parasite tissue become very tough and rigid when preserved in ethanol, using these structures for species identification without additional treatment is difficult. We investigated two different techniques to digest the surrounding tissues, the first was treatment with 10% sodium dodecyl sulphate (SDS) and the second treatment was proteinase K.
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