On-line drug metabolites generation and their subsequent target analysis by capillary zone electrophoresis with UV-absorption detection

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Authors

ZEISBERGEROVÁ Marta ŘEMÍNEK Roman MÁDR Aleš GLATZ Zdeněk HOOGMARTENS Jos VAN SCHEPDAEL Ann

Year of publication 2010
Type Article in Periodical
Magazine / Source Electrophoresis
MU Faculty or unit

Faculty of Science

Citation
Field Biochemistry
Keywords CE; Cytochrome P450; Dextromethorphan; Metabolism;
Description This study presents the in-capillary enzymatic biotransformation of dextromethorphan, an antitusive drug and opioid receptors antagonist, and subsequent electrophoretic separation of its products. The study includes the optimization of separation parameters to fulfill the requirements of an online microreaction. The analyses were performed in a bare fused-silica capillary using 100mM sodium tetraborate (pH 10.0) mixed with linear polyacrylamide (20%, v/v) and 2-propanol (10%, v/v). This BGE was suitable for monitoring both off-line and in-capillary incubations. The partial filling technique enabled the enzymatic reaction to be carried out in its optimal environment (20mM sodium phosphate, pH 7.4). Finally, in-capillary microreaction in the presence of cytochrome P450 3A4 gave satisfactory outcomes.
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