The Nse3/MAGE and Nse4/EID proteins are involved in transcription regulation

• Authors: PALEČEK Jan; BEDNÁŘOVÁ Kateřina; HUDSON Jessica; KOZÁKOVÁ Lucie; GUÉRINEAU Marc; LIAO Chunyan; COLNAGHI Rita; MAREK Jaromír; VIDOT Susanne; LEHMANN Alan
• Year of publication: 2010
• Type: Conference abstract
• MU Faculty or unit: Faculty of Science
• Description: The SMC5/6 complex is composed of two subcomplexes SMC5-SMC6-Nse2 and Nse1-Nse3-Nse4. Nse3 shows sequence similarity to the MAGE (Melanoma Antigen Gene) family of proteins, which are overexpressed in certain types of cancers and whose function has been linked to cell cycle regulation, apoptosis and neuronal development. Using site-directed mutagenesis, protein-protein interaction analyses and modelling of the Schizosaccharomyces pombe Nse3 onto the crystal structure of the MAGEA4 protein, we have identified surfaces on the C- and N-terminal domains of Nse3 that interact respectively with Nse4 and Nse1. The binding site for Nse4 is evolutionarily conserved within the Nse3/MAGE superfamily of proteins. Nse4 is related to the EID (E1A-like inhibitor of differentiation) family of transcriptional repressors. We show that there is interaction between members of the MAGE family and those of the EID family. Our data suggest that the Nse3/MAGE and Nse4/EID proteins are involved in gene regulation.

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