Modulation of gp130 Signalling by Inhibitors of Reactive Oxygen Species Formation in Mouse Embryonic Stem Cells
| Authors | |
|---|---|
| Year of publication | 2009 |
| Type | Article in Periodical |
| Magazine / Source | FREE RADICAL BIOLOGY AND MEDICINE |
| MU Faculty or unit | |
| Citation | |
| Field | Physiology |
| Keywords | stem cells; gp130; reactive oxgen species; ROS; hypoxia; redox modulators; |
| Description | The gp130 system is a set of ligands and receptors that dominantly involves the activation of the JAK/STAT, Erk, and PI3K/Akt pathways. gp130 is suggested to play a key role in response of stem cells to both the mediators that maintain stem cell undifferentiated phenotype and the mediators inducing differentiation. Under physiological conditions in their niche, stem cells are kept in hypoxic microenvironment. Leaving their niche they are exposed to normal levels of oxygen. Thus reactive oxygen species (ROS) formation in stem cells due to hypoxic conditions could be considered as important regulator of stem cell fate. Here we analyzed effects of inhibitors of NADPH oxidases apocynin or diphenyleneiodonium (DPI), and a glutathione precursor N-acetyl-cysteine (NAC) on activity of gp130 signaling pathways and maintenance of mouse ES cells. The obtained results are compared with effects of ES cells culture in hypoxia condition. |
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