Nový bakteriocín rodu Yersinia.

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Title in English New bacteriocin of Yersinia genus.
Authors

BOSÁK Juraj ŠMAJS David

Year of publication 2009
Type Conference abstract
MU Faculty or unit

Faculty of Medicine

Citation
Description In a cross-test experiment, we identified producents of antibacterial agents. Bacteriocin of Y. fredericksenii 27601 was further analyzed by methods of molecular biology. The identified bacteriocin of Y. fredericksenii 27601 is a protein with specific antibacterial activity on bacteria belonging to the related strains of the genus Yersinia. Bacteriocin and immunity protein encoding genes were identified on the plasmid pYF27601 using transposon mutagenesis approach. Plasmid pYF27601 showed high degree of homology to Col-plasmids and its sequencing revealed gene encoding bacteriocin (1317 bp) and immunity protein gene (338 bp). Sequence analysis of the bacteriocin gene revealed homology to genes of alveicin A and alveicin B, colicins Ia and Ib, S-type pyocin and colicin E1. The above described bacteriocin of Y. fredericksenii is a new type of bacteriocin. We propose to denominate it as colicin FY.
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