SUV39h-independent association of HP1b with fibrillarin-positive nucleolar regions

Warning

This publication doesn't include Faculty of Economics and Administration. It includes Faculty of Informatics. Official publication website can be found on muni.cz.
Authors

HARNIČAROVÁ Andrea BÁRTOVÁ Eva GALIOVÁ Gabriela UHLÍŘOVÁ Radka MATULA Pavel KOZUBEK Stanislav

Year of publication 2010
Type Article in Periodical
Magazine / Source Chromosoma
MU Faculty or unit

Faculty of Informatics

Citation
Web http://www.springerlink.com/content/03353h53h300j70k/
Doi http://dx.doi.org/10.1007/s00412-009-0252-2
Field Genetics and molecular biology
Keywords SUV39h; heterochromatin protein 1; nucleoli; cell nucleus
Description Heterochromatin protein 1 (HP1), which binds to sites of histone H3 lysine 9 (H3K9) methylation, is primarily responsible for gene silencing and the formation of heterochromatin. We observed that HP1beta is located in both the chromocenters and fibrillarin-positive nucleoli interiors. However, HP1alfa and HP1gamma occupied fibrillarin-positive compartments to a lesser extent, corresponding to the distinct levels of HP1 subtypes at the promoter of rDNA genes. Deficiency of histone methyltransferases SUV39h, and/or inhibition of histone deacetylases (HDACi) decreased HP1beta and H3K9 trimethylation at chromocenters, but not in fibrillarin-positive regions that co-localized with RNA Pol I. Similarly, SUV39h- and HDACi-dependent nucleolar rearrangement and inhibition of rDNA transcription did not affect the association between HP1beta - and fibrillarin. The presence of HP1beta in nucleoli is likely connected with transcription of ribosomal genes and with the role of fibrillarin in nucleolar processes.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.