Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern hybridization

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Authors

BOMBAROVÁ Marta VÍTKOVÁ Magda ŠPAKULOVÁ Marta KOUBKOVÁ Božena

Year of publication 2009
Type Article in Periodical
Magazine / Source Genome
MU Faculty or unit

Faculty of Science

Citation
Web https://www.nrcresearchpress.com/doi/abs/10.1139/G09-063#.XutX32gzZaQ
Doi http://dx.doi.org/10.1139/G09-063
Field Zoology
Keywords Acanthocephala; Platyhelminthes; chromosomes; FISH; telomere
Description We examined the composition of telomeres in chromosomes of parasitic worms, representatives of the flatworm groups Monogenea and Cestoda (Platyhelminthes), and thorny-headed worms (Syndermata: Acanthocephala) by fluorescence in situ hybridization (FISH) with different telomeric repeat probes. Our results show that the (TTAGGG)n sequence, supposed to be the ancestral telomeric repeat motif of Metazoa, is conserved in Monogenea (Paradiplozoon homoion) and Cestoda (Caryophyllaeus laticeps, Caryophyllaeides fennica, and Nippotaenia mogurndae) but not in Acanthocephala (Pomphorhynchus laevis and Pomphorhynchus tereticollis). In the Pomphorhynchus species, no hybridization signals were obtained with the nematode (TTAGGC)n, arthropod (TTAGG)n, and bdelloid (TGTGGG)n telomeric probes using FISH with their chromosomes and Southern hybridization with P. laevis DNA. Therefore, we suggest that parasitic Acanthocephala have evolved yet unknown telomeric repeat motifs or different mechanisms of telomere maintenance.
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