Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern hybridization

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Authors	BOMBAROVÁ Marta VÍTKOVÁ Magda ŠPAKULOVÁ Marta KOUBKOVÁ Božena
Year of publication	2009
Type	Article in Periodical
Magazine / Source	Genome
MU Faculty or unit	Faculty of Science
Citation
Web	https://www.nrcresearchpress.com/doi/abs/10.1139/G09-063#.XutX32gzZaQ
Doi	http://dx.doi.org/10.1139/G09-063
Field	Zoology
Keywords	Acanthocephala; Platyhelminthes; chromosomes; FISH; telomere
Description	We examined the composition of telomeres in chromosomes of parasitic worms, representatives of the flatworm groups Monogenea and Cestoda (Platyhelminthes), and thorny-headed worms (Syndermata: Acanthocephala) by fluorescence in situ hybridization (FISH) with different telomeric repeat probes. Our results show that the (TTAGGG)n sequence, supposed to be the ancestral telomeric repeat motif of Metazoa, is conserved in Monogenea (Paradiplozoon homoion) and Cestoda (Caryophyllaeus laticeps, Caryophyllaeides fennica, and Nippotaenia mogurndae) but not in Acanthocephala (Pomphorhynchus laevis and Pomphorhynchus tereticollis). In the Pomphorhynchus species, no hybridization signals were obtained with the nematode (TTAGGC)n, arthropod (TTAGG)n, and bdelloid (TGTGGG)n telomeric probes using FISH with their chromosomes and Southern hybridization with P. laevis DNA. Therefore, we suggest that parasitic Acanthocephala have evolved yet unknown telomeric repeat motifs or different mechanisms of telomere maintenance.
Related projects:	Phenotypic plasticity of monogenean parasites: a combined molecular and morphological approach