Structural and functional characterization of lectin AFL1 from Aspergillus fumigatus

• Authors: KOMÁREK Jan; KOSTLÁNOVÁ Nikola; VARROT Annabelle; IMBERTY Anne; WIMMEROVÁ Michaela
• Year of publication: 2009
• Type: Article in Proceedings
• Conference: XIII. Setkání biochemiků a molekulárních biologů
• MU Faculty or unit: Faculty of Science
• Field: Biochemistry
• Keywords: lectin Aspergillus fumigatus
• Description: This study deals with structural and functional characterization of 34.6 kDa lectin AFL1 from ascomycete Aspergillus fumigatus. AFL1 was expressed in a recombinant form and purified by FPLC on mannose-agarose column. Binding properties of AFL1 were determined by SPR and ITC. Several promising crystals for X-ray diffraction have been obtained using hanging drop vapour diffusion technique combined with streak seeding technique.

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