Sequencing and molecular typing of hypervariable loci in the Treponema pallidum genomes: characterizations of type strains and clinical isolates.
| Authors | |
|---|---|
| Year of publication | 2008 |
| Type | Article in Proceedings |
| Conference | Clinical Microbiology and Infection, Volume 14, Issue s7, Abstracts of the 18th ECCMID, P1704 |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | Treponema pallidum; molecular typing |
| Description | Using Comparative Genomic Sequencing (CGS) approach, hypervariable regions in Treponema pallidum genome were identified. These regions comprised hypothetical genes TP0136 and TP0548. Nucleotide sequences of these genes were determined and compared in 4 groups of T. pallidum strains: in ssp. pallidum (6 strains causing syphilis), in ssp. pertenue (6 strains causing yaws), in ssp. endemicum (2 strains causing endemic syphilis) and in 1 strain of unclassified simian isolate. In addition, 9 PCR positive clinical samples were analyzed.The TP0136 and TP0548 analyzed loci showed discrete clusters in 4 groups of type strains. Analysis of strains belonging to ssp. pallidum revealed two discrete subclusters containing Nichols-like and SS14-like sequences, respectively. All clinical isolates clustered with the SS14-like subgroup.Our results showed that TP0136 and TP0548 loci represent promising targets for molecular typing of pathogenic treponemes including clinical samples. |
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