Identification of antifungal proteins in the barley and malt caryopsis by mass spectrometry
| Authors | |
|---|---|
| Year of publication | 2008 |
| Type | Article in Proceedings |
| Conference | Vitamins 2008 Nutrition and Diagnostics |
| MU Faculty or unit | |
| Citation | |
| Field | Analytic chemistry |
| Keywords | low molecular weight proteins; barley; malt; SDS-PAGE; enzymatic digestion; MALDI-TOF mass spectrometry |
| Description | Recently, great attention is paid to determination of various metabolites after pathogen-caryopsis interaction. Some of antifungal proteins, e.g. thionins, might be of effect on barley quality and its subsequent food products - gushing or premature yeast flocculation. The changes proceeding during pathogen-caryopsis interaction were monitored in 20 samples of barley and its corresponding malt by the combination of Sodium Dodecyl Sulphate - Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Matrix-Assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry (MALDI-TOF MS). |
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