IZOLACE A DETEKCE mRNA ZE STRESOVANÝCH BUNĚK POMOCÍ PARAMAGNETICKÝCH ČÁSTIC

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Title in English IZOLATION AND DETECTION OF TRANSCRIPTOME FROM STRESSED CELLS BY USE OF PARAMAGNETIC PARTICLES
Authors

HÚSKA Dalibor ADAM Vojtěch MEIJA Jhonny BALOUN Jiří TRNKOVÁ Libuše HUBÁLEK Jaromír KIZEK René

Year of publication 2008
Type Article in Proceedings
Conference VIII. pracovní setkání fyzikálních chemiků a elektrochemiků. Sborník příspěvků
MU Faculty or unit

Faculty of Science

Citation
Web http://cheminfo.chemi.muni.cz/ktfch/setkani2008.htm
Field Biochemistry
Keywords mRNA; optimization; electrochemical detection
Description Optimization of izolation and detection of mRNA were performed with use of poly(A). Paramagnnetic Dynabeads particles were applied for izolation and electrochemical method square wave voltammetry for mRNA detection. Both methods were found suitable for izolation and detection of transcriptome in plant tissues.
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