IZOLACE A DETEKCE mRNA ZE STRESOVANÝCH BUNĚK POMOCÍ PARAMAGNETICKÝCH ČÁSTIC

• Title in English: IZOLATION AND DETECTION OF TRANSCRIPTOME FROM STRESSED CELLS BY USE OF PARAMAGNETIC PARTICLES
• Authors: HÚSKA Dalibor; ADAM Vojtěch; MEIJA Jhonny; BALOUN Jiří; TRNKOVÁ Libuše; HUBÁLEK Jaromír; KIZEK René
• Year of publication: 2008
• Type: Article in Proceedings
• Conference: VIII. pracovní setkání fyzikálních chemiků a elektrochemiků. Sborník příspěvků
• MU Faculty or unit: Faculty of Science
• Web: http://cheminfo.chemi.muni.cz/ktfch/setkani2008.htm
• Field: Biochemistry
• Keywords: mRNA; optimization; electrochemical detection
• Description: Optimization of izolation and detection of mRNA were performed with use of poly(A). Paramagnnetic Dynabeads particles were applied for izolation and electrochemical method square wave voltammetry for mRNA detection. Both methods were found suitable for izolation and detection of transcriptome in plant tissues.

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