Automated confocal in vivo microscopy based on spinning disks
| Authors | |
|---|---|
| Year of publication | 2005 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | The successful development of non-invasive quantitative visualization techniques for live cell imaging have led to the development of suitable hardware and software for the acquisition and processing of multidimensional image data. Confocal spinning disk systems (based either on a classical Nipkow disk or on the microlens principle) are especially suitable for in vivo imaging thanks to high acquisition speed (parallel imaging of thousands of points), high quality image detection (up to 90% quantum efficiency at negligible noise levels with state-of-the-art cameras), low photobleaching and low phototoxicity. Lately, we have been working on the optimization and automation of image acquisition and processing for this type of microscopy. Special attention has been paid to ultra-fast image acquisition mode. This poster presents both hardware as well as software approach used in our laboratory. It also shows examples of biological tasks that are being solved using the automated confocal in vivo microscopy systems. |
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