Multiplex panels of polymorphic microsatellite loci for two cryptic bat species of the genus Pipistrellus, developed by cross-species amplification within the family Vespertilionidae

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Authors

KAŇUCH Peter FORNŮSKOVÁ Alena BARTONIČKA Tomáš BRYJA Josef

Year of publication 2007
Type Article in Periodical
Magazine / Source Molecular Ecology Notes
MU Faculty or unit

Faculty of Science

Citation
Field Zoology
Keywords Chiroptera microsatellites pipistrellus pygmaeus sibling species
Description The paper presents multiplex panels of polymorphic microsatellites for two closely related cryptic species Pipistrellus pipistrellus and Pipistrellus pygmaeus. We tested the cross-species amplification of 34 microsatellite loci, originally developed for five vespertilionid bat species. Ten and nine polymorphic loci in P. pipistrellus (mean number of alleles per locus = 10.5) and P. pygmaeus (8.1), respectively, in three multiplex PCRs per species were amplified. All loci can be analysed in a single fragment analysis and can be used as markers to the study of evolution and the ecology of structured populations of socially living bats.
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