Fluorescence detection system for capillary separations utilizing a liquid core waveguide with an optical fibre-coupled compact spectrometer
| Authors | |
|---|---|
| Year of publication | 2005 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Chromatography A |
| MU Faculty or unit | |
| Citation | |
| Field | Analytic chemistry |
| Keywords | fluorescence detection; liquid core waveguide; capillary liquid separation methods |
| Description | A simple fluorescence detection system based on liquid core waveguides is described. A fibre-coupled CCD spectrometer installed in a PC is used in this system for detection of the emitted light. The functionality of this system was demonstrated on isoelectric focusing of low-molecular-mass pI markers and FITC derivatized proteins. The ability to acquire emission spectra was presented. The construction, assembling and optical alignment are surprisingly effortless and no other optical components are necessary. The system could be easily tuned for the detection of analytes with different spectral characteristics only by changing the excitation source and without any modification in the detection part. Described detection system also offers a high degree of miniaturization. |
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