Fluorescence detection system for capillary separations utilizing a liquid core waveguide with an optical fibre-coupled compact spectrometer

Investor logo

Warning

This publication doesn't include Faculty of Economics and Administration. It includes Faculty of Science. Official publication website can be found on muni.cz.
Authors

KOŠŤÁL Vratislav ZEISBERGEROVÁ Marta ŠLAIS Karel KAHLE Vladislav

Year of publication 2005
Type Article in Periodical
Magazine / Source Journal of Chromatography A
MU Faculty or unit

Faculty of Science

Citation
Field Analytic chemistry
Keywords fluorescence detection; liquid core waveguide; capillary liquid separation methods
Description A simple fluorescence detection system based on liquid core waveguides is described. A fibre-coupled CCD spectrometer installed in a PC is used in this system for detection of the emitted light. The functionality of this system was demonstrated on isoelectric focusing of low-molecular-mass pI markers and FITC derivatized proteins. The ability to acquire emission spectra was presented. The construction, assembling and optical alignment are surprisingly effortless and no other optical components are necessary. The system could be easily tuned for the detection of analytes with different spectral characteristics only by changing the excitation source and without any modification in the detection part. Described detection system also offers a high degree of miniaturization.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.