Isolation of PCR-ready DNA from real samples containing PCR inhibitors
| Authors | |
|---|---|
| Year of publication | 2007 |
| Type | Article in Proceedings |
| Conference | Biopowders; Advances in the Powder Technology |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | fungi; Trichophyton; nonporous magnetic hydrophilic microspheres; P(HEMA-co-EDMA); P(HEMA-co-GMA) |
| Description | Hydrophilic magnetic particles coated by carboxyl groups were used for PCR-ready DNA isolation. DNA was adsorbed on magnetic particles in the presence of 8 wt. % of poly(ethylene glycol) (PEG 6,000) and 2 M NaCl concentrations, washed and eluted into TE buffer (10 mM Tris-HCl, pH 7.8; 1 mM EDTA; pH 8.0). The eluted DNA was used directly for PCR amplification and identification of Lactobacillus and Bifidobacterium cells in dairy products (cheese, fermented dairy products) and lyophilisates, Trichophyton cells in mixed cultures and bacterial cells in sediments from Lake Baikal. It was also used in GMO testing in foods with success. |
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