Identifikace a stanovení příbuznosti probiotických druhů bakterií rodu Bifidobacterium pomocí molekulárně diagnostických metod

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Title in English Identification and determination of relatioships of Bifidobacterium probiotic strains using molecular diagnostic methods
Authors

KRULOVÁ Blanka ŠPANOVÁ Alena RITTICH Bohuslav

Year of publication 2007
Type Article in Proceedings
Conference XI. Setkání biochemiků a molekulárních biologů
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords Bifidobacterium; PCR; rep-PCR
Description The aim of the work was the use of species specific PCRs for the identification of bacterial species of genus Bifidobacterium isolated from stool of suckling babies. The other aim was the use of rep-PCR for PCR/fingerprinting of studied strains. Species specific PCRs were carried out with 10 different primer pairs. All isolates were indentified as of genus Bifidobacterium, 3 isolates were identified as species B. bifidum, 3 isolates were identified as species B. animalis, 6 isolates were identified as species B. breve, 2 isolates were identified as species B. adolescentis. Other molecular biology methods will be used for confirmation of the above mentioned results
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