Detection of synthetic oligonucleotides by alternating current voltammetry at solid amalgam surfaces.

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Authors	HASOŇ Stanislav VETTERL Vladimír
Year of publication	2006
Type	Article in Periodical
Magazine / Source	Electrochimica Acta
MU Faculty or unit	Faculty of Science
Citation
Field	Electrochemistry
Keywords	solid amalgam electrodes; mercury film electrodes; oligonucleotides; adsorption of oligonucleotides; surface morphology ; glassy carbon electrode; mercury-film electrode;
Description	The tensammetric responses of different synthetic oligonucleotides (ODNs) by an alternating current (AC) voltammetry at the platinum solid amalgam electrode (PtAE)are investigated. The optimalisation of the AC voltammetric measurement of ODNs includes the determination of the effects of: adsorption time (t(A)), concentration of analysed sample (c), thickness of the amalgam layer (h), and use of different solid amalgams such as platinum, copper, gold and silver on the intensity of the AC voltammetric peaks 1 (desorption of ODN segments adsorbed via ODN sugar-phosphate backbone) and 3 (desorption and/or reorientation of the ODN segments adsorbed through hydrophobic base residues) of adsorbed ODNs. The features of mercury modified graphite/carbon electrodes for measurements of tensammetric responses of ODNs are briefly discussed.
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