NMR of Nucleic Acids - Little by Little

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Authors

SKLENÁŘ Vladimír PŘECECHTĚLOVÁ Jana MUNZAROVÁ Markéta FIALA Radovan

Year of publication 2006
Type Article in Proceedings
Conference XXII International Conference on Magnetic Resonance in Biological Systems
MU Faculty or unit

Faculty of Science

Citation
Field Biophysics
Keywords NMR; nucleic acids;DFT;direct detection
Description Nuclear Magnetic Resonance (NMR) spectroscopy is experiencing significant development with new hardware and software tools as well as new methodological progress. The lecture will focus on the theoretical study of 31P NMR parameters in nucleic acids, namely on the dependence the chemical shielding tensor and J-coupling constants on the torsion angles and solvent patterns along the sugar-phosphate backbone in B-DNA. In the second part, applicability of 13C direct detection methods to aid the assignment procedures and structure determination of nucleic acids will be discussed.
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