Re-exprese nestinu v nádorově transformovaných buňkách: in vitro studie na vybraných typech nádorů

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Title in English Nestin re-expression in transformed cells: in vitro study on selected types of human solid tumors
Authors

LOJA Tomáš CHLAPEK Petr ZITTERBART Karel VESELSKÁ Renata

Year of publication 2006
Type Article in Proceedings
Conference II. Dny diagnostické, prediktivní a experimentální onkologie
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords nestin; intermediate filaments; cytoskeleton; neuroblastoma; malignant fibrous histiocytoma; osteoplastic osteosarcoma; glioblastoma multiforme
Description Differences in the cell-specific expression of intermediate filament proteins in neoplastic tissue represent an important tool for tumor diagnostic. According to published studies, the re-expression of nestin has been detected in tumors derived from CNS tissues, as well as in some other types of human solid tumors. It is evident that nestin re-expression in tumor cells is closely related both to the dedifferentiation and malignancy of the respective tumors. Therefore, immunohistochemical and/or immunocytochemical examination of nestin may serve as a useful tool for classification and accurate grading of human malignancies. Our study was focused on a detection of nestin in cell lines derived from several types of human solid tumors. By employing indirect immunofluorescence, we proved a strong re-expression of nestin in NB-01 (neuroblastoma), JE-03 (malignant fibrous histiocytoma), JE-04 (osteoplastic osteosarcoma) and GM-7 (glioblastoma multiforme) cell lines. A detailed study of the nestin expression both on intracellular and ultrastructural levels was performed using GM-7 cell line; the results shown the presence of nestin not only in the cytoplasm but also in the cell nucleus.
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