Molekulárně-biologická diagnostika spirochety Treponema pallidum v biologickém materiálu.
| Title in English | Molecular detection and subtyping of Treponema pallidum subsp. pallidum in clinical specimens |
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| Authors | |
| Year of publication | 2006 |
| Type | Article in Proceedings |
| Conference | X. Pracovní setkání biochemiků a molekulárních biologů. Sborník příspěvků. |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | Treponema pallidum subsp. pallidum; detection in clinical specimens; molecular typing; mucocutaneous swabs. |
| Description | A nested PCR amplification protocol detecting tmpC gene (TP0319, encoding putative membrane lipoprotein) was used for detection of Treponema pallidum subsp. pallidum in the clinical material. We tested 138 samples of blood sera of 111 adult patients in primary, secondary, early and late latent stages of the infection as well as of patients suspected of syphilis. T. p. pallidum DNA was not detected in any of the analyzed sera samples. Out of 11 mucocutaneous swabs (7 genital and 4 pharyngeal ones), 6 from 3 patients with primary or secondary syphilis were positive. In addition, one skin swab was positive in patient with early congenital syphilis. Sera and cerebrospinal fluid samples from this patient were also positive. DNA sequencing of genes TP0136 and TP0548 in the positive samples revealed two patients with DNA sequence identical to T. p. pallidum strain SS14 and two unique, not yet known T. p. pallidum strains. The advances in molecular typing of T. p. pallidum in clinical material will become important for epidemiologic consequences and for clinical discrimination between reinfection and reactivation of the syphilitic process. |
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