Studium promotorů Treponema pallidum: charakterizace promotorů pro faktor sigma-54.
| Title in English | Promoters of Treponema pallidum: promoters for sigma-54. |
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| Authors | |
| Year of publication | 2006 |
| Type | Article in Proceedings |
| Conference | X. Pracovní setkání biochemiků a molekulárních biologů. Sborník příspěvků. |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | T. pallidum ssp. pallidum; promoter; sigma-54 |
| Description | The spirochete T. pallidum ssp. pallidum is the causative agent of syphilis, a sexually transmitted disease characterized by multiple stages and infection of different organs. It was hypothesized that T. pallidum regulates its gene expression during infection of humans. We have studied the function of sigma-54 factor (RpoN) and sigma-54-dependent promoters. Sigma factors inititate transcription by binding to promoter and to RNA-polymerase. The sigma-54-dependent promoters were identified by the PromScan software. pCS26 plasmid was used for the construction of the transcriptional fusions between the promoters and luxCDABE genes. This reporter system was used for verification of identified promoters. Six possible sigma-54-dependent promoters were identified in the genome of T. pallidum. |
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