Thermostable trypsin conjugates for high-throughput proteomics: synthesis and performance evaluation

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Authors	HAVLIŠ Jan ŠEBELA Marek ŠTOSOVÁ Tatiana WIELSCH Natalie THOMAS Henrik ZDRÁHAL Zbyněk SHEVCHENKO Andrej
Year of publication	2006
Type	Article in Periodical
Magazine / Source	Proteomics
MU Faculty or unit	Faculty of Science
Citation
Field	Biochemistry
Keywords	Bovine trypsin; Conjugate; In-gel digestion; Oligosaccharide; PMF
Description	Conjugating bovine trypsin with oligosaccharides maltotriose, raffinose and stachyose increased its thermostability and suppressed autolysis, without affecting its cleavage specificity. These conjugates accelerated the digestion of protein substrates both in solution and in gel, compared to commonly used unmodified and methylated trypsins.
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