Thermostable trypsin conjugates for high-throughput proteomics: synthesis and performance evaluation

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Authors

HAVLIŠ Jan ŠEBELA Marek ŠTOSOVÁ Tatiana WIELSCH Natalie THOMAS Henrik ZDRÁHAL Zbyněk SHEVCHENKO Andrej

Year of publication 2006
Type Article in Periodical
Magazine / Source Proteomics
MU Faculty or unit

Faculty of Science

Citation
Field Biochemistry
Keywords Bovine trypsin; Conjugate; In-gel digestion; Oligosaccharide; PMF
Description Conjugating bovine trypsin with oligosaccharides maltotriose, raffinose and stachyose increased its thermostability and suppressed autolysis, without affecting its cleavage specificity. These conjugates accelerated the digestion of protein substrates both in solution and in gel, compared to commonly used unmodified and methylated trypsins.
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