OXIDATIVNÍ STRES JAKO NEGATIVNÍ PROGNOSTICKÝ FAKTOR U SPINOCELULÁRNÍHO KARCINOMU HLAVY A KRKU
| Title in English | Oxiadtive stress as negative prognostication factor in HNSCC |
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| Authors | |
| Year of publication | 2006 |
| Type | Article in Proceedings |
| Conference | Sborník abstrakt, XXX. Brněnské onkologické dny |
| MU Faculty or unit | |
| Citation | |
| Field | ORL, ophthalmology, stomatology |
| Keywords | oxidative stress; malondialdehyde |
| Description | Introduction: Oxidative status plays an important role in cancer development. Our aim is to investigate relationship between genetic variants encoding for antioxidant enzymes; activity of the expressed enzyme and clinical stage of head and neck squamous cell carcinoma (HNSCC). Material and Methods: prospective study of 65 patients with HNSCC. PCR-based methodology was used to detect genotype of the manganese superoxide dismutase (MnSOD) Ala16Val polymorphism. We measured activity of superoxide dismutase in plasma (pSOD) and in erythrocytes (ercSOD). Using high performance liquid chromatography we analyzed malondialdehyde (MDA) in plasma. Tumor necrosis factor a (TNF) in plasma was measured by the ultrasensitive ELISA. Results: The mean activities of pSOD, resp. ercSOD, were 11117 3102U/g, resp. 4137 821U/g. The mean levels of MDA, resp. TNF, were 2.27 0.67nmol/g, resp. 2.48 1.13ng/L. Val/Val variant manifested higher TNF than other two variants (p<0.05). Increased plasmatic MDA was found in patients with recurrent disease (p<0.05). Patients with negative neck (N0) had lower pSOD activity than those with locoregional metastatic spread (N+) (p<0.0005). Activity of ercSOD and plasmatic level of MDA tended to increase in N+ with no significant difference. Conclusions: Increased MDA resulting from elevated oxidative burden correlates with negative prognosis of a recurrent disease. Similarly, SOD is predisposed to become a prognostic factor in HNSCC since it is significantly increased in N+ patients. This might be caused by stronger tendency to breaks in DNA, resp. formation or progression of HNSCC, in patients with decreased antioxidative defenses or in patients who had encountered massive doses of reactive oxygen species in their previous lives. Homozygotes with Val in MnSOD Ala16Val polymorphism manifested increased TNF. Hence, they were more sensitive to conditions leading to higher TNF levels, e.g. oxidative stress. The project is supported by grant 10/IINR/7996-3 from IGA of The Czech Ministry of Health |
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