Electrochemical immunosensor for detection of Francisella tularensis

Warning

This publication doesn't include Faculty of Economics and Administration. It includes Faculty of Science. Official publication website can be found on muni.cz.
Authors

SKLÁDAL Petr SYMERSKÁ Yveta POHANKA Miroslav ŠAFÁŘ Bohuslav MACELA Aleš

Year of publication 2005
Type Chapter of a book
MU Faculty or unit

Faculty of Science

Citation
Description The immunosensor for rapid and sensitive detection of Francisella tularensis (Ft) was developed using screen-printed sensors with multiple (4) Au working electrodes. The sensing zones were modified with a biolayer of anti-Ft polyclonal antibody attached through the covalently immobilized Protein A. The sandwich assay format was employed with anti-Ft IgG-peroxidase conjugate as tracer. For electrochemical measurements, an automated portable detector was developed; it consisted of a digitally controlled potentiostat and four embedded miniperistaltic pumps. The flow-through measurement involved sequential incubations with sample (10 min), tracer (10 min) and substrate mixture (iodide and hydrogen peroxide). The enzymatically-produced iodine was detected electrochemically at - 50 mV. The limit of detection was 100 Ft cells/ml; the response was significantly higher (3x) when using the living cells compared to the heat inactivated ones.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.