Mapování genomů patogenních spirochet.
| Title in English | Genoe mapping of pathogenic spirochetes |
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| Authors | |
| Year of publication | 2005 |
| Type | Article in Proceedings |
| Conference | Sborník příspěvků IX. Pracovního setkání biochemiků a molekulárních biologů |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | Treponema pallidum ssp. pallidum; Treponema pallidum ssp. pertenue; Treponema paraluiscuniculi |
| Description | Genomes of closely related treponemes, Treponema pallidum ssp. pallidum (strains Nichols and SS14) and Treponema pallidum ssp. pertenue (Gauthier, Samoan D) were compared with the genome of Treponema paraluiscuniculi using genomic techniques. Whole genome fingerprinting was used to study treponemal genomes. PCR-amplified regions of genomic DNA were digested with BamH I, EcoR I, Hind III, or their combinations. PCR amplification and subsequent restriction analysis of the complete genomes of the strains Nichols, SS14 and Gauthier revealed 1 absent restriction site in SS14 and Gauthier. When compared to Nichols genome, 8 and 34 differences in the target restriction sites and 1 and 8 detectable deletions/insertions were found in the genome of Samoan D and T. paraluiscuniculi, respectively. |
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