Fractionation of Iodine in Iodine-enriched Algae Chlorella
| Authors | |
|---|---|
| Year of publication | 2005 |
| Type | Article in Proceedings |
| Conference | International Conference on Environmental Inorganic Analysis - Proceedings of the 4th Conference |
| MU Faculty or unit | |
| Citation | |
| Field | Analytic chemistry |
| Keywords | Iodine; algae; Chlorella; fractionation; speciation |
| Description | Iodine as an essential element has a great importance in human and animal health. Concerning animal husbandry, feed fortified by iodine provides adequate supply of this element and influences the health of animals and the quality of production. The bioavailability of iodine depends on the form of iodine. This work deals with determination of iodine forms in iodine-enriched algae Chlorella, which is cultivated in Institute of Microbiology (Czech Academy of Science) and consecutively applied in in-vivo experiments at University of Veterinary and Pharmaceutical Sciences. Fractionation protocol including sequential extraction by chloroform, water and sodium dodecylsulphate solution was designed. Inductively coupled plasma optical emission spectrometry was employed for determination of iodine in fractions solubilized with TMAH. Iodides were quantified in aqueous extract using vapour-generation ICP-OES after separation of iodides on anion exchange resin. Fractionation gives information on the content of iodine in non-polar, protein, polar water-soluble and non-soluble organic fractions and iodides. Methodology was applied in analysis of twelve algae samples that were cultivated with different cultivating conditions (hetero- or autotrophic). Results of this research are used in optimization of cultivation conditions to get desired composition of iodine-enriched Chlorella. |
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