Energy coupling to nitrate uptake into the denitrifying cells of Paracoccus denitrificans

Warning

This publication doesn't include Faculty of Economics and Administration. It includes Faculty of Science. Official publication website can be found on muni.cz.

Authors	KUČERA Igor
Year of publication	2005
Type	Article in Periodical
Magazine / Source	Biochimica et Biophysica Acta Bioenergetics
MU Faculty or unit	Faculty of Science
Citation
Web	http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T1S-4GTVW4N-1&_user=835458&_handle=V-WA-A-W-AU-MsSAYWW-UUA-U-AABWWUCCCU-AAWEDYZBCU-VAUEDAYAC-AU-U&_fmt=full&_coverDate=09%2F05%2F2005&_rdoc=3&_orig=browse&_srch=%23toc%234898%232005%23982909997%2
Field	Biochemistry
Keywords	nitrate transport denitrification bacteria energization ionophores
Description	This study deals with the effects of the agents that dissipate the individual components of the proton motive force (short-chain fatty acids, nigericin, and valinomycin) upon the methyl viologen-coupled nitrate reductase activity in intact cells. The data indicate that nitrate transport relies heavily on the pH gradient at neutral pH. A potential dependent step may be of significance in the transporter cycle under alkaline pH. Experiments with sodium-depleted media provided no hints for Na+ as a possible H+ substitute.
Related projects:	Proteins in metabolism and interaction of organisms with the environment