Preliminary study on the monitoring of glutathione S-tranferase activity toward styrene oxide by electromigration methods
| Authors | |
|---|---|
| Year of publication | 2005 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Separation Science |
| MU Faculty or unit | |
| Citation | |
| Field | Biochemistry |
| Keywords | styrene oxide; GST; MEKC; CEC |
| Description | A new method has been developed for the monitoring of glutathione S-tranferase (GST) detoxification activity toward styrene oxide (SO). The enzymatic reaction was carried out directly in thermostatted autosampler vial and the formation of conjugates between glutathione (GSH) and SO was monitored by sequential MEKC runs. The determinations were performed in a 50 um fused silica capillary using 50 mM SDS in 20 mM phosphate 20 mM tetraborate buffer (pH 8.3) as a background electrolyte; separation voltage 28 kV (positive polarity), temperature of capillary 25 oC and detection at 200 nm. The method is rapid, able to be automated, and requires only small amount of samples, which is especially important in the case of GST isoenzyme analyses. |
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