Amperometric screen-printed biosensor arrays with co-immobilised oxidoreductases and cholinesterases

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Authors

SOLNÁ Renáta DOCK Eva CHRISTENSON Alexander WINTER-NIELSEN Margrethe CARLSSON Christine EMNÉUS Jenny RUZGAS Tautgirdas SKLÁDAL Petr

Year of publication 2005
Type Article in Periodical
Magazine / Source Analytica Chimica Acta
MU Faculty or unit

Faculty of Science

Citation
Field Biochemistry
Keywords amperometric multienzyme sensor; steady state system; cholinesterase; oxidoreductase; phenoks; pesticides; wastewater; multivariate-analysis
Description Amperometric screen-printed biosensor arrays for detection of pesticides (organophosphates and carbamates) and phenols have been developed. Cholinesterases (AChE and BChE), tyrosinase (TYR), peroxidases (SBP. soybean and HRP. horseradish) and cellobiose dehydrogenase (CDH) were combined on the same array consisting of one Ag/AgCl reference electrode surrounded by eight radially distributed working electrodes of either carbon or platinum. Mainly cross-linking with glutaraldehyde was employed for enzyme immobilisation. The substrates for the enzymes were acetylthiocholine for cholinesterases (ChEs), cellobiose for CDH and hydrogen peroxide for peroxidases. Hydrogen peroxide was generated in the presence of glucose by co-immobilised glucose oxidase (GOx). All measurements were performed in an electrochemical steady state system specially constructed for eight channel screen-printed electrode arrays. The achieved relative standard deviation values calculated for different enzyme substrates (10 measurements) were typically below 7% and one assay was completed within less than 10 min. The detection limits for pesticides and phenols were in the nanomolar and micromolar ranges, respectively. The developed biosensor array was evaluated on wastewater samples. To simplify interpretation of results. the measured data were created with multivariate analysis-principal component analysis (PCA).
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