Stripping voltammetry of oligonucleotides
| Authors | |
|---|---|
| Year of publication | 2004 |
| Type | Article in Proceedings |
| Conference | Book of Abstracts I - 55th Annual Meeting of ISE |
| MU Faculty or unit | |
| Citation | |
| Field | Electrochemistry |
| Keywords | Oligonucleotides; oxidation signals; carbon electrodes; aduct of purines with copper(II) |
| Description | Oligonucleotides are electroactive species producing oxidation signals at carbon electrodes as well as reduction and tensammetric signals at liquid mercury and solid amalgam electrodes. The bases of nucleic acids can be determined by cathodic stripping voltammetry at nanomolar concentrations. Quite recently, Farias et al.presented determination of some nucleic bases in the presence of copper(II) in combination of mercury electrode to their ultratrace determination. Acid treated oligonucleotides containing purine bases were analysed by voltammetric techniques including differential pulse and cyclic voltammetry. The analysis is based on the electrochemical determination of released purine bases in the presence of copper(II) ions. Voltammetric stripping analysis was performed in alkaline or acidic medium using a hanging mercury drop electrode or a carbon paste electrode, respectively. We studied hydrolysed oligonucleotides in dependence on their length and we found good correlation between the voltammetric signal and oligonucleotide length. |
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