Nestin expression in cell lines derived from high-grade astrocytomas.

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Authors

VESELSKÁ Renata KUGLÍK Petr CEJPEK Pavel HUBLAROVÁ Pavla ŠVÁCHOVÁ Hana RELICHOVÁ Jiřina

Year of publication 2004
Type Article in Proceedings
Conference International Journal of Mol. Medicine 14 (Sup. 1) - the 9th World Congress on Advances in Oncology and the 7th International Symposium on Molecular Medicine
MU Faculty or unit

Faculty of Medicine

Citation
Field Oncology and hematology
Keywords nestin; intermediate filaments; immunocytochemical and cytogenetic analysis; astrocytomas
Description Two cell lines were established from biopsy samples of patients surgically treated for glioblastoma multiforme. Genetic abnormalities were studied on fresh bioptic material using interphase fluorescence in situ hybridization (iFISH); cultivated cell lines were analyzed by G-banding and spectral karyotyping (SKY). The expression of intermediate filament proteins (vimentin, GFAP, nestin) was analyzed at different passages for both cell lines.
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