Immunofluorescence staining of the insulin-like growth factor-I in the ventral and dorsal spinal roots

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Authors	SVÍŽENSKÁ Ivana DUBOVÝ Petr KLUSÁKOVÁ Ilona
Year of publication	2002
Type	Article in Periodical
Magazine / Source	Scripta medica
MU Faculty or unit	Faculty of Medicine
Citation
Field	Neurology, neurosurgery, neurosciences
Keywords	Schwann cells; IGF-I; regeneration; peripheral nerve; motor; sensory axons; Lucia G
Description	To reveal whether there are differences in appearance of IGF-I in microenvironment of efferent and afferent axons authors have analysed immunohistochemically the expression of the IGF-I molecules in the intact ventral and dorsal roots of the spinal cord in rat. The results of immunostaining were viewed and digitised in a Leica-DMBL fluorescence microscope equipped with a DC-100 camera. A brightness of fluorescence immunolabeling was measured by means of an image analyzing system (Lucia G). The results revealed a higher IGF-I immunoreactivity in the dorsal roots than in the ventral ones.
Related projects:	Cellular and extracellular environment for the axon regenaration following injury of the spinal roots and peripheral nerves Plasticity of Regulatory Mechanisms of the Central Nervous Systems