Immunofluorescence staining of the insulin-like growth factor-I in the ventral and dorsal spinal roots

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Authors

SVÍŽENSKÁ Ivana DUBOVÝ Petr KLUSÁKOVÁ Ilona

Year of publication 2002
Type Article in Periodical
Magazine / Source Scripta medica
MU Faculty or unit

Faculty of Medicine

Citation
Field Neurology, neurosurgery, neurosciences
Keywords Schwann cells; IGF-I; regeneration; peripheral nerve; motor; sensory axons; Lucia G
Description To reveal whether there are differences in appearance of IGF-I in microenvironment of efferent and afferent axons authors have analysed immunohistochemically the expression of the IGF-I molecules in the intact ventral and dorsal roots of the spinal cord in rat. The results of immunostaining were viewed and digitised in a Leica-DMBL fluorescence microscope equipped with a DC-100 camera. A brightness of fluorescence immunolabeling was measured by means of an image analyzing system (Lucia G). The results revealed a higher IGF-I immunoreactivity in the dorsal roots than in the ventral ones.
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