Magnetic hydrophilic methacrylate-based polymer microspheres designed for polymerase chain reaction applications

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Authors

ŠPANOVÁ Alena HORÁK Daniel SOUDKOVÁ Eva RITTICH Bohuslav

Year of publication 2004
Type Article in Periodical
Magazine / Source Journal of Chromatography B
MU Faculty or unit

Faculty of Science

Citation
Field Microbiology, virology
Keywords Polymerase chain reaction inhibition; Magnetic microspheres; Methacrylates
Description Magnetic hydrophilic nonporous P(HEMA-co-EDMA), P(HEMA-co-GMA) and PGMA microspheres were prepared by dispersion (co)polymerization of HEMA and EDMA or GMA in the presence of several kinds of magnetite.It was found that some components used in the preparation of magnetic carriers interfered with PCR. DNA isolated from bacterial cells of Bifidobacterium longum was used in PCR evaluation of non-interfering magnetic microspheres.Preferably, electrostatically stabilized colloidal magnetite (ferrofluid) should be used in the design of new magnetic mathacrylate-based microspheres by dispersion polymerization.
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