Magnetic hydrophilic methacrylate-based polymer microspheres designed for polymerase chain reaction applications

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Authors	ŠPANOVÁ Alena HORÁK Daniel SOUDKOVÁ Eva RITTICH Bohuslav
Year of publication	2004
Type	Article in Periodical
Magazine / Source	Journal of Chromatography B
MU Faculty or unit	Faculty of Science
Citation
Field	Microbiology, virology
Keywords	Polymerase chain reaction inhibition; Magnetic microspheres; Methacrylates
Description	Magnetic hydrophilic nonporous P(HEMA-co-EDMA), P(HEMA-co-GMA) and PGMA microspheres were prepared by dispersion (co)polymerization of HEMA and EDMA or GMA in the presence of several kinds of magnetite.It was found that some components used in the preparation of magnetic carriers interfered with PCR. DNA isolated from bacterial cells of Bifidobacterium longum was used in PCR evaluation of non-interfering magnetic microspheres.Preferably, electrostatically stabilized colloidal magnetite (ferrofluid) should be used in the design of new magnetic mathacrylate-based microspheres by dispersion polymerization.
Related projects:	The use of magnetic carriers with immobilized selective ligands in DNA diagnostics Development of methods for evaluation and rapid identification of selected lactic acid bacteria using molecular biology methods