Electrophoretically mediated microanalysis (Review)

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Authors

VYTISKOVÁ Soňa VAN DYCK Sigrid VAN SCHEPDAEL Ann HOOGMARTENS Jos GLATZ Zdeněk

Year of publication 2004
Type Article in Periodical
Magazine / Source Journal of Chromatography A
MU Faculty or unit

Faculty of Science

Citation
Field Biochemistry
Keywords Capillary electrophoresis; enzymes; EMMA
Description This review describes the existing developments in the use of the capillary electrophoretic microanalytical technique for the in-line study of enzyme reaction, electrophoretically mediated microanalysis (EMMA). The article is divided into a number of parts. After an introduction, the different modes, basic principle, procedure, and some mathematical treatments of EMMA methodology are discussed and illustrated. The applications of EMMA for enzyme assay and for non-enzymatic determination are summarized into two tables. In addition to classical capillary electrophoresis (CE) instrument EMMA, special emphasis is given to a relatively new technique: EMMA on CE microchip. Finally, conclusions are drawn.
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