Eliminační voltametrie oligonukleotidů

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Title in English	Elimination voltammetry of oligonucleotides
Authors	TRNKOVÁ Libuše POSTBIEGLOVÁ Irena
Year of publication	2003
Type	Article in Periodical
Magazine / Source	CHEMICKÉ LISTY
MU Faculty or unit	Faculty of Science
Citation
Field	Electrochemistry
Keywords	Elimination voltammetry with linear scan (EVLS); homo-and hetero-deoxyoligonucleotides; adsorptive stripping procedure; biosensor
Description	Homo- and hetero-deoxyoligodeoxynukleotides (ODNs), including hairpins, were studied by elimination voltammetry with linear scan (EVLS). Beside the reduction signals of adenine and cytosine the oxidation signals of guanine on HMDE were observed. The registered adenine and cytosine reduction peaks are overlapped. Common voltammetric methods (LSV, CV, SWV, DPV) are not able to resolute these peaks, but EVLS in connection with adsorptive stripping (AdS) recorded two separated peaks. Various experimental conditions have been shown that the effect on the separation of adenine a cytosine signals has not only pH, concentration of substances, but also structure and sequence of nucleic bases in ODNs. New connection of two methods - AdS EVLS provides a promising tool not only for electroanalytical determination of ODNs, but also for the monitoring of structure and hybridization changes. The paper was supported by grants: GA ČR 203/02/0422 a GA AV A 1163201.
Related projects:	Electrodes modified with nucleic acids and proteins. New tools in biochemical and biomedical research New trends in electrochemistry of nucleic acids and their applications in enviromental chemistry Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.